PCR-BASED DETECTION OF A RARE LINEAR DNA IN CELL CULTURE

PCR-based detection of a rare linear DNA in cell culture

The described method allows for detection of rare linear DNA fragments generated during genomic deletions.The predicted limit of the detection is one DNA molecule per 107 or more cells.The method is based on anchor PCR and involves gel separation of the linear DNA fragment and chromosomal DNA before amplification.The detailed chemical structure har

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Influence of Brexit on education towards Europeanism

The present research study explores the extent to which the withdrawal of the UK from the European Union may negatively affect the efforts to lead in particular young people to have a positive attitude towards this integration group, enhance a feeling of belonging to it or a certain sense of common European citizenship.The main aim of the paper was

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Discovery of a new family of relaxases in Firmicutes bacteria.

Antibiotic resistance is a serious global problem.Antibiotic resistance genes (ARG), which are widespread in environmental bacteria, can be transferred to pathogenic bacteria via horizontal gene transfer (HGT).Gut microbiomes are especially apt for the emergence and dissemination of ARG.Conjugation is the HGT route that is predominantly responsible

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